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Chemical Synthesis Services
Publications
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Gel Filtration of Protected Peptides on Sephadex
G-50 in Hexamethylphosphoramide Containing 5% Water
I.J. Galpin, G.W. Kenner, S.R. Ohlsen and
R. Ramage, J. Chromatography, 1975, 106,
125.
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A New Reagent for Polypeptide Synthesis, m-Oxo-bis-[tris-
(dimethylamino)-phosphonium]-bis-tetrafluoroborate
A.J. Bates, I.J. Galpin, A. Hallet, D. Hudson,
G.W. Kenner, R. Ramage and R.C. Sheppard, Helv. Chim. Acta.,
1975, 58, 688.
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Diphenylphosphinamides: Synthesis of Secondary Amines and N-Methylaminoacid
Derivatives
S. Coulton, G.A. Moore and R. Ramage, Tetrahedron
Letters, 1976, 4005.
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Synthesis of Human Big GastrinA.M. Choudhury, G.W.
Kenner, S. Moore, R. Ramage, P.M. Richards, W.D. Thorpe, L. Moroder, G.
Wendelberger and E. Wunsch, Peptides, Proc 14th European
Peptide Symposium, 1976, (Editions of
the University of Brussels), 257.
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Studies on the Synthesis of 1-75 Fragment of a Lysozyme Analogue
I.J. Galpin, B.K. Handa, D. Hudson, A.G. Jackson,
G.W. Kenner, S.R. Ohlsen, R. Ramage, B. Singh and R.G. Tyson, Peptides,
Proc. 14th European Peptide Symposium, 1976,(Editions
of the University of Brussels), 247.
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Purification of Protected Peptides by GPC
I.J. Galpin, B.K. Handa, G.W. Kenner, S. Moore and
R. Ramage, Chromatography of Synthetic and Biological Polymers,
(Ellis Harwood, 1978), 1, 331.
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Improved Method of Gel Filtration of Protected Peptides Using Sephadex LH-60
I.J. Galpin, A.G. Jackson, G.W. Kenner, P. Noble
and R. Ramage, J. Chromatography,
1978, 147, 424.
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Minigastrin: Corrected Structure and Synthesis
R.A. Gregory, A.J. Tracy, J.I. Harris, M.J.
Runswick. S. Moore, G.W. Kenner and R. Ramage, Z. Physiol.
Chem., 1979, 36, 73.
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N-Terminal Sequence of Porcine Big Gastrin: Sequence, Synthesis and
Immunochemical Studies
A.M. Choudhury, K.Y. Chu, G.W. Kenner, S. Moore,
K.L. Ramachandran and R. Ramage, Bioorganic Chem.,
1979, 8, 471
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The Use of Phenyl Esters in Peptide Synthesis
I.J. Galpin, P.M. Hardy, G.W. Kenner, J.R.
McDermott, R. Ramage, J.H. Seely and R.G. Tyson, Tetrahedron,
1979, 35, 2577
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Synthesis of a Polypeptide Chain Comprising 129 Residues. Strategy and Tactics
G.W. Kenner, R. Ramage and R.C. Sheppard,
Tetrahedron, 1979, 35, 2577.
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Synthesis of the 1-16 Fragment of a Lysozyme Analogue
I.J.Galpin, F.E. Hancock, B.K. Handa, A.G.
Jackson, G.W. Kenner, R. Ramage and B. Singh, Tetrahedron,
1979, 35, 2771.
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Synthesis of the 17-26 Fragment of a Lysozyme Analogue
I.J. Galpin, F.E. Hancock, B.K. Handa, A.G.
Jackson, G.W. Kenner, R. Ramage, B. Singh and R.G. Tyson, Tetrahedron,1979,
35, 2779.
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Synthesis of the 27-37 Fragment of a Lysozyme Analogue
I.J. Galpin, G.W. Kenner, S.R. Ohlsen, R. Ramage,
R.C. Sheppard and R.G. Tyson, Tetrahedron,
1979, 35, 2785.
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Synthesis of the 1-37 Fragment of a Lysozyme Analogue
I.J. Galpin, F.E. Hancock, G.K. Handa, A.G.
Jackson, G.W. Kenner, R. Ramage and B. Singh, Tetrahedron,
1979, 35, 1791.
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Synthesis of the 76-129 Fragment of a Lysozyme Analogue
I.J. Galpin, G.W. Kenner, R. Ramage, K.Y. Chu, A.
Hallett, D. Hudson, B.A. Morgan, P. Noble, J. Seely and W.D. Thorpe,
Peptides Proc. 15th European Peptide Symposium,
1978, (Wroclaw University Press, 1979), 665.
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The Use of Immunochemical Methods in Structural Studies on Human Big Gastrin
G.J. Dockray, R.A. Gregory, G.W. Kenner, S. Moore,
K.L. Ramachandran and R. Ramage, Gut Peptides,
A. Miyoshi (Ed), (Kodansha Ltd., Tokyo, 1979), 38
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Synthetic Studies Directed Towards the Synthesis of a Lysozyme Analogue
G.W. Kenner, I.J. Galpin and R. Ramage, Proc.
6th American Peptide Symposium, 1979,
E. Gross and J. Meienhofer (Eds), (Pierce Chemical Co., 1980), 431.
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Corrected Structure and Synthesis of Minigastrin
S. Moore, G.W. Kenner, R. Ramage, R.A. Gregory,
H.J. Tracy, I. Harris and M.J. Runswick, Proc. 6th American
Peptide Symposium, 1979, E. Gross
and J. Meienhofer (Eds), (Pierce Chemical Co., 1980), 503.
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Synthesis of the 38-49 Fragment of a Lysozyme Analogue
I.J. Galpin, G.W. Kenner and R. Ramage, Tetrahedron,
1980, 36, 2241.
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Synthesis of the 50-67 Fragment of a Lysozyme Analogue
I.J. Galpin, B.K. Handa, G.W. Kenner, S.R. Ohlsen
and R. Ramage, Tetrahedron, 1980,
36, 2247.
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Synthesis of the 68-75 Fragment of a Lysozyme Analogue
I.J. Galpin, D. Hudson, A.G. Jackson, G.W. Kenner
and R. Ramage, Tetrahedron, 1980,
36, 2255.
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Synthesis of the 38-75 Fragment of a Lysozyme Analogue
I.J. Galpin, B.K. Handa, A.G. Jackson, G.W.
Kenner, P. McDowell, S.R. Ohlsen and R. Ramage, Tetrahedron,1980,
36, 2259.
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NH2-Terminal Sequence of Human Big Gastrin Sequence. Synthetic and
Immunochemical Studies
A.M. Choudhury, G.W. Kenner, S. Moore, L.K.
Ramachandran, W.D. Thorpe, R. Ramage, G.J. Dockray, R.A. Gregory, L. Hood and
M. Hunkapiller, Z. Physiol. Chem.,
1980, 361, 1719.
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Porcine Big Gastrin: Sequence, Synthesis and Immuno-chemical Studies
G.W. Kenner, S. Moore, K.L. Ramachandran, R.
Ramage, G.J. Dockray, R.A. Gregory, L. Hood and M. Hunkapiller, Bioorganic
Chem., 1981, 10, 152.
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Synthesis of the 76-93 Fragment of a Lysozyme Analogue
I.J. Galpin, A. Hallett, G.W. Kenner, P. Noble, R.
Ramage and J.H. Seely,Tetrahedron,
1981, 37, 3017.
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Synthesis of the 94-104 Fragment of a Lysozyme Analogue
I.J. Galpin, D. Hudson, G.W. Kenner, P. Noble and
R. Ramage, Tetrahedron, 1981, 37,
3025.
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Synthesis of the 105-117 Fragment of a Lysozyme Analogue
I.J. Galpin, G.W. Kenner, B.A. Morgan, P. Noble
and R. Ramage, Tetrahedron, 1981,
37, 3031.
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Synthesis of the 118-129 Fragment of a Lysozyme Analogue
I.J. Galpin, G.W. Kenner, R. Ramage and W.D.
Thorpe, Tetrahedron, 1981, 37,
3037.
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Studies in the Synthesis of an Analogue of Hen Egg White Lysozyme
I.J. Galpin, F.E. Hancock, B.K. Handa, A.G.
Jackson, G.W. Kenner, P. McDowell, P. Noble and R. Ramage, Tetrahedron,
1981, 37, 3043.
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Design of Organophosphorus Reagents for Peptide Synthesis
R. Ramage, B. Atrash and M.J. Parrott, ACS
Symposium Series No. 171, Phosphorus Chemistry,
Quin and Verkade (Eds), (The American Chemical Society, 1981), 199.
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Application of Phosphinic Acids to Peptide Synthesis
R. Ramage, C.P. Ashton, B. Atrash, D. Hopton and
M.J. Parrott, Peptides,
1982, K. Blaha and P. Malon (Eds), (Walter de Gruyter, Berlin, 1983), 157.
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Phosphinamides: A New Class of Amino Protecting Groups in Peptide Synthesis
R. Ramage, D. Hopton, M.J. Parrott, G.W. Kenner
and G.A. Moore, J. Chem. Soc. (Perkin I),
1984, 1357.
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The Application of Diphenylphosphinic-Carboxylic Mixed An-hydrides to Peptide
Synthesis
R. Ramage, D. Hopton, M.J. Parrott and R.S.
Richardson, J.Chem. Soc. (Perkin I),
1985, 461.
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Evaluation of Phosphinic Acid Derivatives as Reagents for Amine Protection in
Peptide Synthesis
R. Ramage, B. Atrash, D. Hopton and M.J. Parrott,
J. Chem.Soc. (Perkin I), 1985, 1217.
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A Kinetic Study of Phosphinic-Carboxylic Anhydrides
R. Ramage, B. Atrash, D. Hopton and M.J. Parrott,
J. Chem. Soc. (Perkin I), 1985,
1617.
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Design of Reagents for Solid Phase Synthesis
R. Ramage, S. Bielecki and M.R. Florence,
Proceedings of the 19th European Peptide Symposium,
1986, 135.
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Application of Mixed Phosphinic Anhydride in Fragment Condensation of Peptides
C. Poulos, Th. Tsegenidis and R. Ramage,
Proceedings of the19th European Peptide Symposium,
1986, 119.
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NG-2,2,7,8-Pentamethylchroman-6-sulphonyl-L-arginine: A New Acid Labile
Derivative for Peptide Synthesis
R. Ramage and J. Green, Tetrahedron
Letters, 1987, 28, 2287.
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Solid Phase Peptide Synthesis: Fluoride Ion Release of Peptide from the Resin
R. Ramage, C.A. Barron, S. Bielecki and D.W.
Thomas, Tetrahedron Letters, 1987,
28, 4105.
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Application of the NG-(2,2,5,7,8-pentamethylchroman-6-sulphonyl) Derivative of
Fmoc-Arginine to Peptide Synthesis
J. Green, O.M. Ogunjobi, R. Ramage, A.S.J.
Stewart, S. McCurdy and R. Noble, Tetrahedron Letters,
1988, 29, 4341.
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Development of Methodology for Arginine and N-alpha Protection in Peptide
Synthesis
R. Ramage, J. Green and M.R. Florence, Proc.
of 10th American Peptide Symposium,1988,
157.
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Solid Phase Peptide Synthesis of Ubiquitin
R. Ramage, J. Green and O.M. Ogunjobi, Tetrahedron
Letters, 1989, 30, 2149.
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Methodology and Strategy in Peptide Synthesis: An Approach to the Synthesis of
Ubiquitin
J. Green. O.M. Ogunjobi and R. Ramage,
Proc. of 20th European Peptide Symposium,
1989, 172.
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The Chemical Synthesis of Proteins: Solid Phase Synthesis of Ubiquitin
R. Ramage, J. Green and O.M. Ogunjobi, in
'Solid Phase Peptide Synthesis', ed. R.
Epton, SPCC (UK), Ltd, Birmingham, 1990, p 27.
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Ubiquitin: Preparative Chemical Synthesis, Purification and Characterisation
O.M. Ogunjobi and R. Ramage, Biochemical
Society Transactions, 1990, 1322.
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An Acid Labile Arginine Derivative for Peptide Synthesis:
NG-2,2,5,7,8-Pentamethylchroman-6-sulphonyl-L-arginine,
R. Ramage, J. Green and A.J. Blake, Tetrahedron,
1991, 47, 6353-6370.
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A Base Labile Protecting Group for Peptide Synthesis: 2,2-
(Bis-(4'-nitrophenyl)ethan-1-oxycarbonyl Urethanes
R. Ramage, A.J. Blake, M.R. Florence, T. Gray, G.
Raphy and P.L. Roach, Tetrahedron,
1991, 47, 8001.
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Chemical Synthesis of Proteins
R. Ramage, Conferencias Plenanias de la
XXIII Reunion bienal de la Real Sociedad Espanola de Quimica,
1991, 101.
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Solid Phase Peptide Synthesis: Fluoride Ion Release of Protected Peptide
Fragments
R. Ramage, C.A. Barron, S. Bielecki, R. Holden and
D.W. Thomas, Tetrahedron, 1992,
48, 499.
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Design of an Affinity-based N -Amino Protecting Group for Peptide Synthesis:
Tetrabenzo[a,c,g,i] Fluorenyl-17-methyl Urethanes (Tbfmoc)
R. Ramage and G. Raphy,Tetrahedron
Letters, 1992, 33, 385.
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Application of Carboxylic-phospholanic Mixed Anhydrides to Fragment Coupling in
Peptide Synthesis
C. Poulos, C.P. Ashton, J. Green, O.M.
Ogunjobi, R. Ramage and T.Tsegenidis, International Journal of Peptide and
Protein Res., 1992, 40, 31.
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Phosphorus-based Reagents in Peptide Synthesis: Synthesis of
Methionne-Enkephalin and the Solution Conformation of its N-Diphenylphosphinoyl
Derivative
D.D. Smith, K.G. Boyd, D. Hopton, R.L. Baxter and
R. Ramage, J. Chem. Soc., Perkin Trans. 1,
1993, 551-556.
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Peptide Synthesis: Synthesis of the Cysteine-containing Peptides of Biological
and Pharmaceutical Interest, a-h-ANF and H-Big Endothelin
R. Ramage and A.S.J. Stewart,J. Chem.
Soc., Perkin Trans. 1, 1993, 1947-1952.
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4-(17-Tetrabenzo[a,c,g,i]fluorenylmethyl)-4'4"-Dimethoxy- trityl Chloride: A
Hydrophobic 5'- Protecting Group for the Separation of Synthetic
Oligonucleotides
R. Ramage and F.O. Wahl, Tetrahedron
Letts., 1993, 34, 7133-7136.
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Design of a Versatile Linker for Solid Phase Peptide Synthesis
R. Ramage, S.L. Irving and C. McInnes, Tetrahedron
Letts., 1993, 34, 6599-6602.
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Affinity Purification of Synthetic Peptides and Proteins on Porous Graphitised
Carbon
A.R. Brown, S.L. Irving and R. Ramage, Tetrahedron
Letts., 1993, 34, 7129-7132.
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Ubiquitin Carboxyl-terminal Hydrolases in Human Brain
R. Layfield, T. Muir, R. Ramage, M.
Landon and R.J. Mayer, J. Neurochem., 1993,
61, 174.
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UV Deprotection Monitoring of an Automated Batch Fmoc Solid Phase Peptide
Synthesis with Feedback Control
K.M. Otteson, R.L. Noble, P.D. Hoeprich, Jr., K.T.
Shaw and R. Ramage,Protein Analysis Renaissance,
69-72.
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Solid Phase Synthesis of a-Hydroxyglycine Extended Peptides - Biological
Precursors of Peptide Amides
A.R. Brown and R. Ramage, Tetrahedron
Letts., 1994, 35, 789-792.
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Synthetic, Structural and Biological Studies of the Ubiquitin System. Part 1.
The Total Chemical Synthesis of Ubiquitin
R. Ramage, J. Green, T.W. Muir, O.M. Ogunjobi, S.
Love and K. Shaw,Biochem. J., 1994,
299, 151-158.
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Synthetic, Structural and Biological Studies of the Ubiquitin System. Part 2.
Chemically Synthesised and Native Ubiquitin Fold into Identical
Three-dimensional Structures
D. Alexeev, S.M. Fawcett, M.A. Turner, O.M.
Ogunjobi, T.W. Muir, R. Ramage and L. Sawyer, Biochem. J.,
1994, 299, 159-163.
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Chemical Synthesis of Proteins: Development of New Methodology
R. Ramage, A.R. Brown, C. McInnes, S.L. Irving,
S.G. Love, W.D. Pallin, T.W. Muir, G. Raphy, K. Shaw, F.O. Wahl and J. Wilken,
in 'Solid Phase Synthesis', ed R. Epton,
(Mayflower Worldwide Ltd, Birmingham), 1994, 1-8.
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Synthesis of 17-Tetrabenzo[a,c,g,i]fluorene Derivatives as Chiral Selectors for
Enantiomeric Separation by HPLC on Porous Graphitised Carbon
J.K. Dutton, J.H. Knox, X Radisson, J.H. Ritchie
and R. Ramage, J. Chem. Soc., Perkin Trans. 1,
1995, 2581-2587.
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(17-Tetrabenzo[a,c,g,i]fluorenyl)methylchloroformate (Tbfmoc Cl) a Reagent for
the Rapid and Efficient Purification of Synthetic Peptides and Proteins
A R Brown, S L Irving, R. Ramage and G. Raphy,
Tetrahedron, 1995, 43, 11815-11830.
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The Impact of Polystyrene Resins in Solid-Phase Organic Synthesis
A.A. MacDonald, S.H. De Witt, S. Ghogh, E.M.
Hogan, L Kieras, A Z Czarnik and R. Ramage, Molecular Diversity,
1995, 1, 183-186.
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The Total Chemical Synthesis of Monocyte Chemotactic Protein-1 (MCP-1)
A.R. Brown, M. Covington, R.C. Newton, R. Ramage
and P. Welch, J. Peptide Science,
1996, 2, 40-46.
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Design and Synthesis of a Highly Immunogenic, Discontinuous Epitope of HIV-1
gp120 which Binds to CD4+ve T Cells
G.J. Cotton, S.E.M. Howie, Ian Heslop, James A
Ross, David J Harrison and R. Ramage, J. Mol. Immunology,
1996, 33, 171-178.
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Methodology for Chemical Synthesis of Proteins
R.Ramage, G.W. Biggin, A.R. Brown, A. Comer, A.
Davison, L. Draffan, Lu Jiang, G. Morton, N. Robertson, K.T. Shaw, G. Tennant,
K. Urquhart and J Wilken, Solid Phase Synthesis,
1996, 1-10
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Synthetic Structural and Biological Studies of the Ubiquitin System: synthesis
and crystal structure of an analogue containing unnatural amino acids
S.G Love, T.W.Muir, R.Ramage, K.T.Shaw, D.Alexeev,
L.Sawyer, S.M.Kelly, N.C.Price, J.E.Arnolds, M.P.Mees and R.J.Mayer, Biochem.J.
1997, 323, 727-734
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Capillary Electrophoresis Assay for Ubiquitin Carboxyl-Terminal Hydrolases with
Chemically Synthesized Ubiquitin-Valine as Substrate
K Franklin, R Layfield, M Landon, R Ramage, A
Brown, S Love, T Muir, K Urquhart, M Bownes, and J Mayer,Analytical
Biochemistry,1997, 247, 305-309
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Solution Structure Determination of Endothelin-1 in Methanol/Water by NMR and
Molecular Modelling Methods
C.M.Hewage, L.Jiang, J.A.Parkinson, R.Ramage, and
I.H.Sadler, Journal of Peptide Science,1997, 3,
415-428
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Solution conformation of an ETB selective agonist, ET-1[Cys(Acm)1,15Ala3,Leu7,Aib11],
in CD3OH/H2O by 1H NMR and molecular modelling
C M Hewage, L Jiang, J A Parkinson, R Ramage and I
H Sadler, FEBS Letters, 1998, 425,
234-238
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Synthetic peptides representing discontinuous CD4 binding
epitopes of HIV-1 gp 120 that induce T cell apoptosis and block cell death
induced by gp 120
S M Howie, G J Cotton, I Heslop, N Martin, D J
Harrison and R Ramage, The FASEB Journal,
1998, 12, 991-998.
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Transfer Active Ester Condensation: A novel technique for peptide segment
coupling
P Wang, R Layfield, M Landon, R J Mayer and R
Ramage, Tetrahedron Letters, 1998,
39, 8711-8714.
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Synthesis of Peptide C-Terminal Derivatives using the Transfer Active Ester
Condensation Technique
P Wang, K T Shaw, B Whigham and R Ramage,
Tetrahedron Letters, 1998, 39, 8719-8720
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A Novel Purification Protocol for Soluble Combinatorial Peptide Libraries
R Ramage, H R Swenson and K T Shaw, Tetrahedron
Letters, 1998, 39, 8715-8718
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Synthesis and Application of a Novel Coupling Reagent, Ethyl 1-Hydroxy-1H -1,2,3
Triazole-4-Carboxylate
L.Jiang, A Davison, G Tennant and R Ramage,
Tetrahedron, 1998, 54, 14233-14254
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Development of ETB Selective Agonists: Solution Structure of a Linear
Endothelin-1 Analogue, ET-1 [Cys(Acm)1,15, Ala3, Leu7,
dAsp8, Aib11]
C M Hewage, L Jiang, J A Parkinson, R Ramage and I
H Sadler, Journal of Biomolecular Structure & Dynamics,
1998, 16, 425-435
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Peptides 1996
Editors R. Ramage and R. Epton, Mayflower,1998
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Selective isopeptide bond formation: Coupling ubiquitin67-76with
histone 2A114 128by use of the transfer active ester condensation
technique
P Wang, R Layfield, M Landon, R J Mayer and R
Ramage, Journal of Peptide Research,
1999, 53, 1-5
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A functional discontinuous HIV-1 gp 120 C3/C4 domain-derived, branched,
synthetic peptide that binds to CD4 an inhibits MIP-1a
chemokine binding
S E M Howie, M L Fernandes, I Heslop, T J Hewson,
G J Cotton, M J Moore, D Innes, R Ramage and D J Harrison, FASEB
Journal, 1999, 13, 503-511
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Total Chemical Synthesis of Deglycosylated Human Erythropoietin
N Robertson, R Ramage, J.Chem.Soc.,
Perkin Trans 1, 1999, 1015-1021
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Comparative Studies of Nsc and Fmoc as Na -protecting
Groups for SPPS
R Ramage, L Jiang, Y D Kim, K Shaw, J Parkand H J
Kim, Journal of Peptide Science,
1999, 5, 195-200
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Racemisation Studies of a Novel Coupling Reagent for Solid Phase Peptide
Synthesis
N Robertson, L Jiang and R Ramage,Tetrahedron,1999,
55, 2713-2720
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Solution Structure of a novel ETB receptor selective agonist
ET1-21[Cys)Acm)1,15, Aib3,11,Leu7] by nuclear
magnetic resonance spectroscopy and molecular modelling
C M Hewage, L Jiang, J A Parkinson, R Ramage, I H
Sadler, Journal of Peptide Research,
1999, 53, 223-233
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Electrophoretic separation of ubiquitin and single amino acid residue ubiquitin
extensions using a commercial modified acrylamide gel electrophoresis system:
An assay to determine catalytic capacities of deubiquitinating enzymes
R Layfield, C Hayes, P Wang, K Urquhart, R Ramage,
R J Mayer, M Landon Electrophoresis,
1999, 20, 480-482
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Solid Phase Synthesis of Peptide C-Terminal Semicarbazones and Aldehydes
J A Patterson and R Ramage,Tetrahedron
letters, 1999, 40, 6121-6124
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Chemically Synthesised Ubiquitin Extension Proteins Detect Distinct Catalytic
Capacities of Deubiquitinating Enzymes
R Layfield, K Franklin, M Landon, G Walker, P
Wang, R Ramage, AR Brown S Love, K Urquhart, T Muir, R T Baker and R J Mayer,
Analytical Biochemistry, 1999, 274,
40-49
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A linear endothelin-1 analogue: solution structure of ET-1[Aib1,3,11,15,
Nle7] by nuclear magnetic resonance spectroscopy and molecular
modelling
C M Hewage, L Jiang, J A Parkinson, R Ramage and I
Sadler, Neurochemistry International,
1999, 35, 35-45
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